Photocyanine is a novel anticancer drug. Its pharmacokinetic study in cancer patients is therefore very important for choosing\ndoses, and dosing intervals in clinical application. A rapid, selective and sensitive high-performance liquid chromatographytandem\nmass spectrometry (HPLC-MS/MS) method was developed and validated for the determination of photocyanine in patient\nserum. Sample preparation involved one-step protein precipitation by adding methanol and N,N-dimethyl formamide to 0.1mL\nserum. The detection was performed on a triple quadrupole tandem mass spectrometer operating in multiple reaction-monitoring\n(MRM) mode. Each sample was chromatographed within 7 min. Linear calibration curves were obtained for photocyanine at a\nconcentration range of 20ââ?¬â??2000 ng/mL (???? > 0.995), with the lower limit of quantification (LLOQ) being 20 ng/mL. The intrabatch\naccuracy ranged from 101.98% to 107.54%, and the interbatch accuracy varied from 100.52% to 105.62%. Stability tests showed\nthat photocyanine was stable throughout the analytical procedure. This study is the first to utilize the HPLC-MS/MS method for\nthe pharmacokinetic study of photocyanine in six cancer patients who had received a single dose of photocyanine (0.1mg/kg)\nadministered intravenously.
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